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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM175B Polyclonal antibody

FAM175B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26868-1-AP

Synonyms

Abraxas brother protein 1, ABRO1, BRISC complex subunit Abro1, FAM175B, KIAA0157, Protein FAM175B

Validation Data Gallery

Applications:
  • WB analysis of HL-60 using 26868-1-AP

    WB analysis of HL-60 using 26868-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 26868-1-AP

    WB analysis of HEK-293 using 26868-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 26868-1-AP

    WB analysis of mouse heart using 26868-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 26868-1-AP

    IHC staining of human heart using 26868-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 26868-1-AP

    IHC staining of human heart using 26868-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHL-60 cells, HEK-293 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26868-1-AP is FAM175B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM175B fusion protein Ag22121 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 175, member B
Calculated molecular weight 415 aa, 47 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC008999
Gene symbol FAM175B
Gene ID (NCBI) 23172
RRIDAB_2880663
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM175B antibody 26868-1-APDownload protocol
IHC protocol for FAM175B antibody 26868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols