Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, HEK-293 cells, mouse heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
26868-1-AP targets FAM175B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM175B fusion protein Ag22121 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 175, member B |
| Calculated molecular weight | 415 aa, 47 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC008999 |
| Gene Symbol | FAM175B |
| Gene ID (NCBI) | 23172 |
| RRID | AB_2880663 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15018 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM175B (also known as ABRO1/KIAA0157) is a scaffold subunit of the BRCC36-containing deubiquitinase complex (BRISC) that specifically cleaves Lys63-linked polyubiquitin chains, thereby modulating DNA-damage responses and receptor trafficking. Originally identified as a binding partner of JunD and ATF4, FAM175B stabilizes ATF4 by inhibiting its ubiquitin-dependent degradation, leading to activation of the ATF4-CHOP pathway and induction of apoptosis under endoplasmic reticulum stress. In esophageal squamous cell carcinoma, FAM175B expression is down-regulated and acts as a p53-independent tumor suppressor; restoring its expression reduces proliferation and increases apoptosis of p53-mutated tumor cells. Collectively, FAM175B serves as a critical scaffold that links deubiquitinase activity to transcriptional and pro-apoptotic programs, offering a potential biomarker for early diagnosis and therapeutic targeting in cancers with p53 dysfunction (PMID: 30854784).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM175B antibody 26868-1-AP | Download protocol |
| IHC protocol for FAM175B antibody 26868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy A bacterial RING ubiquitin ligase triggering stepwise degradation of BRISC via TOLLIP-mediated selective autophagy manipulates host inflammatory response |