• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

FAM171A2 Polyclonal antibody

FAM171A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20836-1-AP

Synonyms

FAM171A2, Protein FAM171A2

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 20836-1-AP

    WB analysis of mouse liver using 20836-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 20836-1-AP

    WB analysis of mouse liver using 20836-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 20836-1-AP

    WB analysis of HepG2 using 20836-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 20836-1-AP

    WB analysis of HepG2 using 20836-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20836-1-AP

    IHC staining of mouse brain using 20836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20836-1-AP

    IHC staining of mouse brain using 20836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20836-1-AP is FAM171A2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM171A2 fusion protein Ag14909 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 171, member A2
Calculated molecular weight 826 aa, 87 kDa
Observed molecular weight 65-75 kDa
GenBank accession numberBC030200
Gene symbol FAM171A2
Gene ID (NCBI) 284069
RRIDAB_2878748
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM171A2 antibody 20836-1-APDownload protocol
IHC protocol for FAM171A2 antibody 20836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IF

Sci Adv

The FAM171A2 gene is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases.

Authors - Wei Xu
  • KD Validated
View Article