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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FAM164C Polyclonal antibody (20520-1-AP)

    WB analysis of HeLa using 20520-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FAM164C Polyclonal antibody (20520-1-AP)

    WB analysis of HeLa using 20520-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of C6 cells using FAM164C Polyclonal antibody (20520-1-AP)

    WB analysis of C6 using 20520-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using FAM164C Polyclonal antibody (20520-1-AP)

    IHC staining of human testis using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAM164C Polyclonal antibody (20520-1-AP)

    IHC staining of human kidney using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAM164C Polyclonal antibody (20520-1-AP)

    IHC staining of human kidney using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using FAM164C Polyclonal antibody (20520-1-AP)

    IHC staining of human testis using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using FAM164C Polyclonal antibody (20520-1-AP)

    IF Staining of C6 using 20520-1-AP

    Immunofluorescent analysis of C6 cells using 20520-1-AP (FAM164C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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FAM164C Polyclonal antibody

FAM164C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20520-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Zinc finger C2HC domain-containing protein 1C, ZC2HC1C, C14orf140

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, C6 cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20520-1-AP targets FAM164C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM164C fusion protein Ag14359 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 164, member C
Calculated molecular weight 275 aa, 31 kDa
Observed molecular weight 31-36 kDa
GenBank accession numberBC004259
Gene Symbol FAM164C
Gene ID (NCBI) 79696
RRIDAB_2878697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53FD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM164C, also named as ZC2HC1C or C14orf140, is a 456 amino acid protein, which contains one C2HC-type zinc finger and belongs to the ZC2HC1 family. FAM164C exists as two isoforms. The isoform1 is a 51kDa protein ad isoform2 is 31kDa.

Protocols

Product Specific Protocols
WB protocol for FAM164C antibody 20520-1-APDownload protocol
IHC protocol for FAM164C antibody 20520-1-APDownload protocol
IF protocol for FAM164C antibody 20520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols