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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM164C Polyclonal antibody

FAM164C Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20520-1-AP

Synonyms

C14orf140, FAM164C, Protein FAM164C

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 20520-1-AP

    WB analysis of HeLa using 20520-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 20520-1-AP

    WB analysis of HeLa using 20520-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • WB analysis of C6 using 20520-1-AP

    WB analysis of C6 using 20520-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20520-1-AP

    IHC staining of human testis using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 20520-1-AP

    IHC staining of human kidney using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 20520-1-AP

    IHC staining of human testis using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 20520-1-AP

    IHC staining of human kidney using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of C6 using 20520-1-AP

    IF Staining of C6 using 20520-1-AP

    Immunofluorescent analysis of C6 cells using 20520-1-AP (FAM164C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, C6 cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20520-1-AP is FAM164C Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM164C fusion protein Ag14359 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 164, member C
Calculated molecular weight 275 aa, 31 kDa
Observed molecular weight 31-36 kDa
GenBank accession numberBC004259
Gene symbol FAM164C
Gene ID (NCBI) 79696
RRIDAB_2878697
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM164C, also named as ZC2HC1C or C14orf140, is a 456 amino acid protein, which contains one C2HC-type zinc finger and belongs to the ZC2HC1 family. FAM164C exists as two isoforms. The isoform1 is a 51kDa protein ad isoform2 is 31kDa.

Protocols

Product Specific Protocols
WB protocol for FAM164C antibody 20520-1-APDownload protocol
IHC protocol for FAM164C antibody 20520-1-APDownload protocol
IF protocol for FAM164C antibody 20520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols