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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FAM160B1 Polyclonal antibody (25388-1-AP)

    WB analysis of PC-3 using 25388-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25388-1-AP (FAM160B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using FAM160B1 Polyclonal antibody (25388-1-AP)

    IP experiment of PC-3 using 25388-1-AP

    IP result of anti-FAM160B1 (IP:25388-1-AP, 5ug; Detection:25388-1-AP 1:300) with PC-3 cells lysate 1500ug.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using FAM160B1 Polyclonal antibody (25388-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25388-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25388-1-AP (FAM160B1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FAM160B1 Polyclonal antibody (25388-1-AP)

    IF Staining of PC-3 using 25388-1-AP

    Immunofluorescent analysis of PC-3 cells using 25388-1-AP (FAM160B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FAM160B1 Polyclonal antibody

FAM160B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25388-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

FHIP2A, FHF complex subunit HOOK interacting protein 2A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25388-1-AP targets FAM160B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22051

Product name: Recombinant human FAM160B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 136-319 aa of BC037207

Sequence: IRLCGEVLATPTENEEIQFLCIVCAKLKQDPYLVNFFLENKMKSLASKGVPNVISEDTLKGQDSLSTDTGQSRQPEELSGATGMEQTELEDEPPHQMDHLSTSLDNLSVTSLPEASVVCPNQDYNLVNSLLNLTRSPDGRIAVKACEGLMLLVSLPEPAAAKCLTQSTCLCELLTDRLASLYKA

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 160, member B1
Calculated molecular weight 765 aa, 86 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC037207
Gene Symbol FAM160B1
Gene ID (NCBI) 57700
RRIDAB_2880054
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5W0V3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM160B1, also named as KIAA1600, is a 765 amino acid protein, which belongs to the UPF0518 family. The biological function of FAM160B1is still non-known. FAM160B1 exists as two isoforms and the molecular weight of isoform one is a 86 kDa protein.The isoforms two is produced by alternative splicing and is a 83 kDa protein. There are modified residues of FAM160B1 protein, so we detected 95 kDa and 100 kDa two bands by western blotting.

Protocols

Product Specific Protocols
WB protocol for FAM160B1 antibody 25388-1-APDownload protocol
IHC protocol for FAM160B1 antibody 25388-1-APDownload protocol
IF protocol for FAM160B1 antibody 25388-1-APDownload protocol
IP protocol for FAM160B1 antibody 25388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols