Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse lung tissue, mouse ovary tissue, mouse pancreas tissue |
| Positive IHC detected in | human skeletal muscle tissue, human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
55401-1-AP targets FAM13A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 13, member A |
| Calculated molecular weight | 117 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | NM_014883 |
| Gene Symbol | FAM13A |
| Gene ID (NCBI) | 10144 |
| RRID | AB_11182723 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94988 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM13A, also named as FAM13A1 and KIAA0914, belongs to the FAM13 family. It is associated with chronic obstructive pulmonary disease.(PMID: 20173748) This antibody is specific to FAM13A. It can recognize all the isoforms of FAM13A.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM13A antibody 55401-1-AP | Download protocol |
| IHC protocol for FAM13A antibody 55401-1-AP | Download protocol |
| WB protocol for FAM13A antibody 55401-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol Annexin A2 Regulates Autophagy in Pseudomonas aeruginosa Infection through the Akt1-mTOR-ULK1/2 Signaling Pathway. | ||
Front Physiol A Protective Role of FAM13A in Human Airway Epithelial Cells Upon Exposure to Cigarette Smoke Extract. | ||
Mol Med Rep miR‑30a‑5p induces the adipogenic differentiation of bone marrow mesenchymal stem cells by targeting FAM13A/Wnt/β‑catenin signaling in aplastic anemia.
| ||
Am J Physiol Lung Cell Mol Physiol FAM13A regulates cellular senescence marker p21 and mitochondrial reactive oxygen species production in airway epithelial cells | ||
Am J Respir Cell Mol Biol AKT Phosphorylates FAM13A and Promotes Its Degradation via CUL4A/DDB1/DCAF1 E3 Complex | ||
Am J Respir Cell Mol Biol The FAM13A Long Isoform Regulates Cilia Movement and Co-ordination in Airway Mucociliary Transport |