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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM136A Recombinant antibody (84150-2-RR)

    WB analysis using 84150-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84150-2-RR (FAM136A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM136A Recombinant antibody (84150-2-RR)

    IHC staining of mouse testis using 84150-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM136A Recombinant antibody (84150-2-RR)

    IHC staining of mouse testis using 84150-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FAM136A Recombinant antibody (84150-2-RR)

    IF Staining of HeLa using 84150-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FAM136A antibody (84150-2-RR, Clone: 241433D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using FAM136A Recombinant antibody (84150-2-RR)

    FC experiment of U2OS using 84150-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug FAM136A Recombinant antibody (84150-2-RR, Clone:241433D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84150-2-RR

    Affinity and Kinetic Characterization of 84150-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84150-2-RR against Human FAM136A were performed. The affinity constant is below 1 pM.

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FAM136A Recombinant antibody

FAM136A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84150-2-RR
Clone No.241433D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

241433D12, Protein FAM136A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cell, Jurkat cell, MCF-7 cell, U2OS cell, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84150-2-RR targets FAM136A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAM136A fusion protein Ag34058 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 136, member A
Observed molecular weight12 kDa
GenBank accession numberBC014975
Gene Symbol FAM136A
Gene ID (NCBI) 84908
RRIDAB_3671715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96C01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM136A is an evolutively conserved nuclear gene encoding a mitochondrial protein whose specific function is unknown, but which is commonly found in neurosensory epithelial cells, where it plays a role in the electron transport chain of respiration (PMID: 37461313). FAM136A, with a molecular mass of 16-kDa, is normally expressed in the cytoplasm and has been linked to familial Meniere's disease.

Protocols

Product Specific Protocols
WB protocol for FAM136A antibody 84150-2-RRDownload protocol
IHC protocol for FAM136A antibody 84150-2-RRDownload protocol
IF protocol for FAM136A antibody 84150-2-RRDownload protocol
FC protocol for FAM136A antibody 84150-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols