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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM136A Polyclonal antibody (30845-1-AP)

    WB analysis using 30845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30845-1-AP (FAM136A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FAM136A Polyclonal antibody (30845-1-AP)

    IHC staining of human ovary cancer using 30845-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30845-1-AP (FAM136A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FAM136A Polyclonal antibody (30845-1-AP)

    IF Staining of U2OS using 30845-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FAM136A antibody (30845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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FAM136A Polyclonal antibody

FAM136A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30845-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein FAM136A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, A549 cells, HeLa cells, Jurkat cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30845-1-AP targets FAM136A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM136A fusion protein Ag34058 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 136, member A
Observed molecular weight15 kDa
GenBank accession numberBC014975
Gene Symbol FAM136A
Gene ID (NCBI) 84908
RRIDAB_3086420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96C01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM136A is an evolutively conserved nuclear gene encoding a mitochondrial protein whose specific function is unknown, but which is commonly found in neurosensory epithelial cells, where it plays a role in the electron transport chain of respiration (PMID: 37461313). FAM136A, with a molecular mass of 16-kDa, is normally expressed in the cytoplasm and has been linked to familial Meniere's disease.

Protocols

Product Specific Protocols
WB protocol for FAM136A antibody 30845-1-APDownload protocol
IHC protocol for FAM136A antibody 30845-1-APDownload protocol
IF protocol for FAM136A antibody 30845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FEBS Open Bio

FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production

Authors - Yushi Otsuka
View Article