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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM135B Polyclonal antibody (28061-1-AP)

    WB analysis using 28061-1-AP (same clone as 28061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28061-1-AP (FAM135B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IHC staining of human kidney using 28061-1-AP (same clone as 28061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IHC staining of human kidney using 28061-1-AP (same clone as 28061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IHC staining of mouse brain using 28061-1-AP (same clone as 28061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IHC staining of mouse brain using 28061-1-AP (same clone as 28061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28061-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IF Staining of mouse brain using 28061-1-AP (same clone as 28061-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FAM135B antibody (28061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28061-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IF Staining of mouse brain using 28061-1-AP (same clone as 28061-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FAM135B antibody (28061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28061-1-PBS in a different storage buffer formulation.

FAM135B Polyclonal antibody, PBS Only

FAM135B Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 28061-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28061-1-PBS targets FAM135B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27897

Product name: Recombinant human FAM135B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 756-1007 aa of BC106912

Sequence: EEDEREVALTKLTKSVSAPHISSPEEAAEDADTKQQDGGFAEPSDMHSKSQGSPGSCSQLCGDSGTDAGADHPLVEIVLDADNQQGPGYIDIPKGKGKQFDAQGHCLPDGRTENTPGVETKGLNLKIPRVIALENPRTRSLHRALEETPKGMPKDLNVGQQALSNSGISEVEGLSQHQVPELSCTSAADAINRNSTGQQSQSGSPCIMDDTAFNRGVNAFPEAKHKAGTVCPTVTHSVHSQVLKNQELKAGT

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 135, member B
Observed molecular weight126-155 kDa
GenBank accession numberBC106912
Gene Symbol FAM135B
Gene ID (NCBI) 51059
RRIDAB_2881050
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ49AJ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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