Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25718-1-AP targets FAM135A in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22472 Product name: Recombinant human FAM135A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC131782 Sequence: MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPSRIPHRVEASLLHATGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSPHRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFIIADSFLHHAYRFHYTLCATLLLAFKGLHSYFITVTEEIPSCQKLELEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRILLAQEHHTLRVRRFSEA 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 135, member A |
| Calculated molecular weight | 1515 aa, 170 kDa |
| GenBank accession number | BC131782 |
| Gene Symbol | FAM135A |
| Gene ID (NCBI) | 57579 |
| RRID | AB_3669496 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2D6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM135A (family with sequence similarity 135) also known as KIAA1411, is a gene in the RP25 locus that is situated between D6S1557 and D6S421. FAM135A is expressed in the retina, brain, skeletal muscle, pancreas, and lung, according to gene expression studies. FAM135A is linked to several tumor-related pathways and is strongly correlated with breast cancer cell invasion and migration (PMID: 36943627).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM135A antibody 25718-1-AP | Download protocol |
| IHC protocol for FAM135A antibody 25718-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |