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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM134C Polyclonal antibody (26330-1-AP)

    WB analysis using 26330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26330-1-AP (FAM134C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FAM134C Polyclonal antibody (26330-1-AP)

    IF Staining of U2OS using 26330-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FAM134C antibody (26330-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FAM134C Polyclonal antibody

FAM134C Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26330-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RETREG3, Reticulophagy regulator 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HuH-7 cellls
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26330-1-AP targets FAM134C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23680

Product name: Recombinant human FAM134C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-127 aa of BC049370

Sequence: MAEAEGVPTTPGPASGSTFRGRRDVSGSWERDQQVEAAQRALVEVLGPYEPLLSRVQAALVWERPARSALWCLGLNAAFWFFALTSLRLVFLLAFGLMIIVCIDQWKNKIWPEIKVPRPDALDNESW

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 134, member C
Observed molecular weight65 kDa
GenBank accession numberBC049370
Gene Symbol FAM134C
Gene ID (NCBI) 162427
RRIDAB_3085859
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VR2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The protein family with sequence similarity 134 (FAM134) comprises three proteins: FAM134B, FAM134A, and FAM134C. These proteins share the same LIR amino acid sequence. FAM134C, also known as the reticulophagy regulator 3 (RETREG3), plays a crucial role as an ER-phagy receptor. It is essential for maintaining ER morphology in a LC3-interacting region (LIR)-dependent manner.

Protocols

Product Specific Protocols
IF protocol for FAM134C antibody 26330-1-APDownload protocol
WB protocol for FAM134C antibody 26330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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