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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM134A Recombinant antibody (85791-4-RR)

    WB analysis using 85791-4-RR (same clone as 85791-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85791-4-RR (FAM134A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85791-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM134A Recombinant antibody (85791-4-RR)

    IHC staining of mouse testis using 85791-4-RR (same clone as 85791-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85791-4-RR (FAM134A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85791-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM134A Recombinant antibody (85791-4-RR)

    IHC staining of mouse testis using 85791-4-RR (same clone as 85791-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85791-4-RR (FAM134A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85791-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85791-4-RR

    Affinity and Kinetic Characterization of 85791-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85791-4-RR against Human FAM134A were performed. The affinity constant is 3.17 nM.

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FAM134A Recombinant antibody, PBS Only

FAM134A Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85791-4-PBS
Clone No.250097C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

C2orf17, MAG2, Protein FAM134A, Reticulophagy regulator 2, RETREG2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85791-4-PBS targets FAM134A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAM134A fusion protein Ag20025 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 134, member A
Calculated molecular weight 543 aa, 58 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC002420
Gene Symbol FAM134A
Gene ID (NCBI) 79137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NC44
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The protein family with sequence similarity 134 (FAM134) consists of three proteins, FAM134B, FAM134A, and FAM134C, which share the same LIR amino acid sequence. FAM134 reticulon family proteins (FAM134A, FAM134B, and FAM134C) are ER-phagy receptors with structural similarities and nonredundant functions. The protein family with sequence similarity 134 (FAM134). Fam134A (also known as metastasis-associated genes 2, MAG-2) is widely expressed in various types of tissues including tumors, and is considered to be closely correlated with tumor proliferation, invasion, and metastasis.