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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM134A Polyclonal antibody (24650-1-AP)

    WB analysis using 24650-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24650-1-AP (FAM134A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM134A Polyclonal antibody (24650-1-AP)

    IHC staining of mouse brain using 24650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24650-1-AP (FAM134A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM134A Polyclonal antibody (24650-1-AP)

    IHC staining of mouse brain using 24650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24650-1-AP (FAM134A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FAM134A Polyclonal antibody (24650-1-AP)

    IF Staining of U2OS using 24650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FAM134A antibody (24650-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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FAM134A Polyclonal antibody

FAM134A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24650-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C2orf17, MAG2, Protein FAM134A, Reticulophagy regulator 2, RETREG2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse thymus tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24650-1-AP targets FAM134A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM134A fusion protein Ag20025 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 134, member A
Calculated molecular weight 543 aa, 58 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC002420
Gene Symbol FAM134A
Gene ID (NCBI) 79137
RRIDAB_3085745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NC44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The protein family with sequence similarity 134 (FAM134) consists of three proteins, FAM134B, FAM134A, and FAM134C, which share the same LIR amino acid sequence. FAM134 reticulon family proteins (FAM134A, FAM134B, and FAM134C) are ER-phagy receptors with structural similarities and nonredundant functions. The protein family with sequence similarity 134 (FAM134). Fam134A (also known as metastasis-associated genes 2, MAG-2) is widely expressed in various types of tissues including tumors, and is considered to be closely correlated with tumor proliferation, invasion, and metastasis.

Protocols

Product Specific Protocols
WB protocol for FAM134A antibody 24650-1-APDownload protocol
IHC protocol for FAM134A antibody 24650-1-APDownload protocol
IF protocol for FAM134A antibody 24650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols