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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM129A Polyclonal antibody (21333-1-AP)

    WB analysis using 21333-1-AP (same clone as 21333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21333-1-AP (FAM129A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FAM129A Polyclonal antibody (21333-1-AP)

    IHC staining of human pancreas cancer using 21333-1-AP (same clone as 21333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21333-1-AP (FAM129A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FAM129A Polyclonal antibody (21333-1-AP)

    IHC staining of human pancreas cancer using 21333-1-AP (same clone as 21333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21333-1-AP (FAM129A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21333-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM129A Polyclonal antibody (21333-1-AP)

    IF Staining of HepG2 using 21333-1-AP (same clone as 21333-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM129A antibody (21333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21333-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FAM129A Polyclonal antibody, PBS Only

FAM129A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21333-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein FAM129A, NIBAN1, NIBAN, GIG39, Cell growth-inhibiting gene 39 protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21333-1-PBS targets FAM129A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15413

Product name: Recombinant human FAM129A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC030531

Sequence: MGGSASSQLDEGKCAYIRGKTEAAIKNFSPYYSRQYSVAFCNHVRTEVEQQRDLTSQFLKTKPPLAPGTILYEAELSQFSEDIKKWKERYVVVKNDYAVESYENKEAYQRGAAPKCRILPAGGKVLTSEDEYNLLSDRHFPDPLASSEKENTQPFVVLPKEFPVYLWQPFFRHGYFCFHEAADQKRFSALLSDCVRHLNHDYMKQMTFEAQAFLEAVQFFRQEKGHYGSWEMITGDEIQILSNLVMEELLPTLQTDLLPKMKGKKNDRKRTWLGLLEEAYTLVQHQVSEGLSALKEECRALTKGLEGTIRSDMDQIVNSKNYLIGKIKAMVAQPAEKSCLESVQPFLASI

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 129, member A
Calculated molecular weight 928 aa, 103 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC030531
Gene Symbol FAM129A
Gene ID (NCBI) 116496
RRIDAB_10733106
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZQ8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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