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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using FAM127A Polyclonal antibody (10307-1-AP)

    WB analysis of human brain using 10307-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10307-1-AP (FAM127A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FAM127A Polyclonal antibody (10307-1-AP)

    IHC staining of human pancreas cancer using 10307-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10307-1-AP (FAM127A antibody) at dilution of 1:100 (under 25x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FAM127A Polyclonal antibody

FAM127A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10307-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

CAAX box protein 1, CXX1, FAM127A, Mar8, MAR8C, Mart8, MART8C, Protein FAM127A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10307-1-AP targets FAM127A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM127A fusion protein Ag0298 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 127, member A
Calculated molecular weight 22 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC002410
Gene Symbol FAM127A
Gene ID (NCBI) 8933
RRIDAB_2101197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6ZKI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAAX box protein 1 is also name as CXX1 or FAM127A. The function of CXX1 remains largely unknown. Catalog#10307-1-AP is a rabbit polyclonal antibody raised against the full-length of human CXX1. Two FAM127A proteins are presented in UniProt: O15255 and A6ZKI3 (UniProt Number). This antibody specially recognises the O15255.

Protocols

Product Specific Protocols
WB protocol for FAM127A antibody 10307-1-APDownload protocol
IHC protocol for FAM127A antibody 10307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Sci

The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency.

Authors - Maiko Yamamoto
View Article