Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
10307-1-AP targets FAM127A in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0298 Product name: Recombinant human FAM127A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 59-182 aa of BC002410 Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 127, member A |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC002410 |
| Gene Symbol | FAM127A |
| Gene ID (NCBI) | 8933 |
| RRID | AB_2101197 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A6ZKI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CAAX box protein 1 is also name as CXX1 or FAM127A. The function of CXX1 remains largely unknown. Catalog#10307-1-AP is a rabbit polyclonal antibody raised against the full-length of human CXX1. Two FAM127A proteins are presented in UniProt: O15255 and A6ZKI3 (UniProt Number). This antibody specially recognises the O15255.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM127A antibody 10307-1-AP | Download protocol |
| IHC protocol for FAM127A antibody 10307-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |