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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FAM127 Polyclonal antibody (20282-1-AP)

    WB analysis of PC-3 using 20282-1-AP (same clone as 20282-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FAM127 Polyclonal antibody (20282-1-AP)

    WB analysis of HeLa using 20282-1-AP (same clone as 20282-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-3 cells using FAM127 Polyclonal antibody (20282-1-AP)

    IP experiment of PC-3 using 20282-1-AP (same clone as 20282-1-PBS)

    IP result of anti-FAM127 (IP:20282-1-AP, 4ug; Detection:20282-1-AP 1:500) with PC-3 cells lysate 3960 ug. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FAM127 Polyclonal antibody (20282-1-AP)

    IHC staining of human colon cancer using 20282-1-AP (same clone as 20282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20282-1-AP (FAM127 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FAM127 Polyclonal antibody (20282-1-AP)

    IHC staining of human colon cancer using 20282-1-AP (same clone as 20282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20282-1-AP (FAM127 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM127 Polyclonal antibody (20282-1-AP)

    IF Staining of HepG2 using 20282-1-AP (same clone as 20282-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using FAM127B antibody 20282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

FAM127 Polyclonal antibody, PBS Only

FAM127 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20282-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

FAM127 A/B/C, CXX1, FAM127A, FAM127B, FAM127C

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20282-1-PBS targets FAM127 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13930

Product name: Recombinant human FAM127B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-113 aa of BC000393

Sequence: MDGRVQLMKALLAGPLRPAARRWRNPIPFPETFDGDTDRLPEFIVQTCSYMFVDENTFSNDALKVTFLITRLTGPALQWVIPYIRKESPLLNDYRGFLAEMKRVFGWEEDEDF

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 127, member B
Calculated molecular weight 113 aa, 13 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC000393
Gene Symbol FAM127B
Gene ID (NCBI) 26071
RRIDAB_10694677
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWD3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.

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