• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM125A Polyclonal antibody

FAM125A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25816-1-AP

Synonyms

CFBP, ESCRT I complex subunit MVB12A, FAM125A, Protein FAM125A

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 25816-1-AP

    WB analysis of Jurkat using 25816-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25816-1-AP (FAM125A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 25816-1-AP

    IHC staining of human pancreas using 25816-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25816-1-AP (FAM125A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 25816-1-AP

    IHC staining of human pancreas using 25816-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25816-1-AP (FAM125A Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 25816-1-AP

    IF Staining of HeLa using 25816-1-AP

    Immunofluorescent analysis of HeLa cells using 25816-1-AP (FAM125A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25816-1-AP is FAM125A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM125A fusion protein Ag22919 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 125, member A
Calculated molecular weight 273 aa, 29 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC007883
Gene symbol FAM125A
Gene ID (NCBI) 93343
RRIDAB_2880250
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM125A, also named as MVB12A and CFBP, is a component of the ESCRT-I complex. FAM125A is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. FAM125A has three isoforms with MW 27-28 kDa and 25 kDa.

Protocols

Product Specific Protocols
WB protocol for FAM125A antibody 25816-1-APDownload protocol
IHC protocol for FAM125A antibody 25816-1-APDownload protocol
IF protocol for FAM125A antibody 25816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols