Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
21313-1-AP targets FAM124B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM124B fusion protein Ag15963 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 124B |
| Calculated molecular weight | 455 aa, 51 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC025754 |
| Gene Symbol | FAM124B |
| Gene ID (NCBI) | 79843 |
| RRID | AB_10859248 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H5Z6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM124B is identified as a novel component of CHD7 and CHD8 containing complex. It is a mainly nuclear localized protein with a widespread expressed. Fam124B is a novel protein possibly involved in the pathogenesis of CHARGE syndrome and neurodevelopmental disorders.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM124B antibody 21313-1-AP | Download protocol |
| IHC protocol for FAM124B antibody 21313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS One Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex. | ||
Oncol Lett New prognostic factors and scoring system for patients with acute myeloid leukemia. | ||
Sci Rep Genome-wide association study of subfoveal choroidal thickness in a longitudinal cohort of older adults |