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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FAM120C Polyclonal antibody (25987-1-AP)

    WB analysis of HepG2 using 25987-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25987-1-AP (FAM120C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using FAM120C Polyclonal antibody (25987-1-AP)

    IP experiment of HepG2 using 25987-1-AP

    IP result of anti-FAM120C (IP:25987-1-AP, 4ug; Detection:25987-1-AP 1:500) with HepG2 cells lysate 3600ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM120C Polyclonal antibody (25987-1-AP)

    IF Staining of HepG2 using 25987-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25987-1-AP (FAM120C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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FAM120C Polyclonal antibody

FAM120C Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 25987-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

CXorf17, FAM120C, ORF34, Protein FAM120C, Tumor antigen BJ HCC 21

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25987-1-AP targets FAM120C in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM120C fusion protein Ag23235 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 120C
Observed molecular weight 120 kDa
GenBank accession numberBC136413
Gene Symbol FAM120C
Gene ID (NCBI) 54954
RRIDAB_2880321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM120C antibody 25987-1-APDownload protocol
IF protocol for FAM120C antibody 25987-1-APDownload protocol
IP protocol for FAM120C antibody 25987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Neuron

Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes

Authors - Norah Al-Azzam
View Article