Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, PC-12 cells, NIH/3T3 cells, A549 cells, HepG2 cells, LNCaP cells, HeLa cells, HSC-T6 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
67926-1-Ig targets FAM114A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17289 Product name: Recombinant human FAM114A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 305-538 aa of BC040452 Sequence: LEILSNESESKVQSFLASLDGEKLELLKNDLISIKDIFAAKELENEENQEEQGLEEKGEEFARMLTELLFELHVAATPDKLNKAMKRAHDWVEEDQTVVSVDVAKVSEEETKKEEKEEKSQDPQEDKKEEKKTKTIEEVYMSSIESLAEVTARCIEQLHKVAELILHGQEEEKPAQDQAKVLIKLTTAMCNEVASLSKKFTNSLTTVGSNKKAEVLNPMISSVLLEGCNSTTYI 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 114, member A1 |
| Calculated molecular weight | 563 aa, 61 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC040452 |
| Gene Symbol | FAM114A1 |
| Gene ID (NCBI) | 92689 |
| RRID | AB_2918678 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IWE2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM114A1 antibody 67926-1-Ig | Download protocol |
| IHC protocol for FAM114A1 antibody 67926-1-Ig | Download protocol |
| WB protocol for FAM114A1 antibody 67926-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |