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Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using FAM114A1 Polyclonal antibody (21638-1-AP)

    WB analysis of Neuro-2a using 21638-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21638-1-AP (FAM114A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM114A1 Polyclonal antibody (21638-1-AP)

    IHC staining of mouse brain using 21638-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21638-1-AP (FAM114A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM114A1 Polyclonal antibody (21638-1-AP)

    IF Staining of HepG2 using 21638-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM114A1 antibody (21638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

FAM114A1 Polyclonal antibody

FAM114A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21638-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FAM114A1, Noxp20, Protein FAM114A1, Protein NOXP20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNeuro-2a cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21638-1-AP targets FAM114A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16287

Product name: Recombinant human FAM114A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 305-538 aa of BC040452

Sequence: LEILSNESESKVQSFLASLDGEKLELLKNDLISIKDIFAAKELENEENQEEQGLEEKGEEFARMLTELLFELHVAATPDKLNKAMKRAHDWVEEDQTVVSVDVAKVSEEETKKEEKEEKSQDPQEDKKEEKKTKTIEEVYMSSIESLAEVTARCIEQLHKVAELILHGQEEEKPAQDQAKVLIKLTTAMCNEVASLSKKFTNSLTTVGSNKKAEVLNPMISSVLLEGCNSTTYI

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 114, member A1
Calculated molecular weight 563 aa, 61 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC040452
Gene Symbol FAM114A1
Gene ID (NCBI) 92689
RRIDAB_2878895
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWE2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.

Protocols

Product Specific Protocols
WB protocol for FAM114A1 antibody 21638-1-APDownload protocol
IHC protocol for FAM114A1 antibody 21638-1-APDownload protocol
IF protocol for FAM114A1 antibody 21638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols