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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using FAM103A1 Polyclonal antibody (19422-1-AP)

    WB analysis of A549 using 19422-1-AP (same clone as 19422-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19422-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using FAM103A1 Polyclonal antibody (19422-1-AP)

    WB analysis of MCF-7 using 19422-1-AP (same clone as 19422-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19422-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using FAM103A1 Polyclonal antibody (19422-1-AP)

    WB analysis of PC-3 using 19422-1-AP (same clone as 19422-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using FAM103A1 Polyclonal antibody (19422-1-AP)

    IHC staining of mouse lung using 19422-1-AP (same clone as 19422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using FAM103A1 Polyclonal antibody (19422-1-AP)

    IHC staining of mouse lung using 19422-1-AP (same clone as 19422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using FAM103A1 Polyclonal antibody (19422-1-AP)

    IHC staining of human oesophagus cancer using 19422-1-AP (same clone as 19422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using FAM103A1 Polyclonal antibody (19422-1-AP)

    IHC staining of human oesophagus cancer using 19422-1-AP (same clone as 19422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19422-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FAM103A1 Polyclonal antibody (19422-1-AP)

    IF Staining of MCF-7 using 19422-1-AP (same clone as 19422-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 19422-1-AP (FAM103A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19422-1-PBS in a different storage buffer formulation.

FAM103A1 Polyclonal antibody, PBS Only

FAM103A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19422-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19422-1-PBS targets FAM103A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13749

Product name: Recombinant human FAM103A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC003627

Sequence: MTDTAEAVPKFEEMFASRFTENDKEYQEYLKRPPESPPIVEEWNSRAGGNQRNRGNRLQDNRQFRGRDNRWGWPSDNRSNQWHGRSWGNNYPQHRQEPYYPQQYGHYGYNQRPPYGYY

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 103, member A1
Calculated molecular weight 118 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC003627
Gene Symbol FAM103A1
Gene ID (NCBI) 83640
RRIDAB_2878578
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTL3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FAM103A1, also named as RNMT-activating mini protein, is a 118 amino acid protein, which belongs to the RAM family. FAM103A1 as a component of mRNA cap binding complex localizes in the nucleus. FAM103A1 is required for efficient mRNA cap methylation and regulates RNMT expression by a post-transcriptional stabilizing mechanism.

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