• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FAK Recombinant antibody (81334-5-RR)

    WB analysis using 81334-5-RR (same clone as 81334-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81334-5-RR (FAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FAK Recombinant antibody (81334-5-RR)

    IF Staining of MCF-7 using 81334-5-RR (same clone as 81334-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FAK Recombinant antibody (81334-5-RR)

    IF Staining of A549 using 81334-5-RR (same clone as 81334-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81334-5-RR

    Affinity and Kinetic Characterization of 81334-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81334-5-RR against Human FAK were performed. The affinity constant is 2.03 nM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FAK Recombinant antibody, PBS Only

FAK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81334-5-PBS
Clone No.242295E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81334-5-PBS targets FAK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAK fusion protein Ag3331 相同性解析による交差性が予測される生物種
Full Name PTK2 protein tyrosine kinase 2
Calculated molecular weight 1052 aa, 119 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC028733
Gene Symbol FAK
Gene ID (NCBI) 5747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05397
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971).