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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAF2 Polyclonal antibody (16251-1-AP)

    WB analysis using 16251-1-AP (same clone as 16251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FAF2 Polyclonal antibody (16251-1-AP)

    WB analysis using 16251-1-AP (same clone as 16251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FAF2 Polyclonal antibody (16251-1-AP)

    WB analysis of HeLa using 16251-1-AP (same clone as 16251-1-PBS)

    WB result of FAF2 antibody (16251-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using FAF2 Polyclonal antibody (16251-1-AP)

    IP experiment of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

    IP result of anti-FAF2 (IP:16251-1-AP, 3ug; Detection:16251-1-AP 1:1000) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAF2 Polyclonal antibody (16251-1-AP)

    IHC staining of human breast cancer using 16251-1-AP (same clone as 16251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAF2 Polyclonal antibody (16251-1-AP)

    IHC staining of human breast cancer using 16251-1-AP (same clone as 16251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAF2 Polyclonal antibody (16251-1-AP)

    IHC staining of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAF2 Polyclonal antibody (16251-1-AP)

    IHC staining of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FAF2 Polyclonal antibody, PBS Only

FAF2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16251-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16251-1-PBS targets FAF2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9236

Product name: Recombinant human FAF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 97-445 aa of BC014001

Sequence: YLIMLPFRFTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNTLCAPEVISLINTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLKDRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRQNLQEEKERKLECLPPEPSPDDPESVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCIPSEEWPNPPTLQEAGLSHTEVLFVQDLTDE

相同性解析による交差性が予測される生物種
Full Name Fas associated factor family member 2
Calculated molecular weight 445 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC014001
Gene Symbol FAF2
Gene ID (NCBI) 23197
RRIDAB_2262469
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CS3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

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