Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31896-1-AP targets FABP9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35224 Product name: Recombinant human FABP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 18-100 aa of NM_001080526 Sequence: EDYMKELGVNFAARNMAGLVKPTVTISVDGKMMTIRTESSFQDTKISFKLGEEFDETTADNRKVKSTITLENGSMIHVQKWLG 相同性解析による交差性が予測される生物種 |
| Full Name | fatty acid binding protein 9, testis |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | NM_001080526 |
| Gene Symbol | FABP9 |
| Gene ID (NCBI) | 646480 |
| RRID | AB_3742489 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q0Z7S8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FABP9 (Fatty Acid-Binding Protein 9) is a cytoplasmic protein and a member of the fatty acid-binding protein superfamily. It is specifically and highly expressed in testicular tissue and plays a critical role in the differentiation of male germ cells. This protein reversibly binds hydrophobic ligands such as fatty acids and retinol, mediating intracellular lipid transport and metabolic regulation. During spermatogenesis, FABP9 maintains the attachment of the acrosome to the sperm nucleus, which is essential for fertilization. Recent studies have revealed that FABP9 is significantly overexpressed in various tumors, including prostate cancer and breast cancer, where it promotes tumor progression through lipid metabolism reprogramming and regulation of the G2/M checkpoint. Its expression is associated with poor patient prognosis, making it a potential diagnostic biomarker and therapeutic target.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FABP9 antibody 31896-1-AP | Download protocol |
| WB protocol for FABP9 antibody 31896-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |