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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FABP3 Monoclonal antibody (60280-1-Ig)

    WB analysis using 60280-1-Ig (same clone as 60280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60280-1-Ig (FABP3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using FABP3 Monoclonal antibody (60280-1-Ig)

    IHC staining of human heart using 60280-1-Ig (same clone as 60280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60280-1-Ig (FABP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using FABP3 Monoclonal antibody (60280-1-Ig)

    IHC staining of human heart using 60280-1-Ig (same clone as 60280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60280-1-Ig (FABP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60280-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using FABP3 Monoclonal antibody (60280-1-Ig)

    IF Staining of mouse heart using 60280-1-Ig (same clone as 60280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60280-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using FABP3 Monoclonal antibody (60280-1-Ig)

    IF Staining of mouse heart using 60280-1-Ig (same clone as 60280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60280-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using FABP3 Monoclonal antibody (60280-1-Ig)

    IF Staining of mouse heart using 60280-1-Ig (same clone as 60280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60280-1-PBS in a different storage buffer formulation.

FABP3 Monoclonal antibody, PBS Only

FABP3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60280-1-PBS
Clone No.2B5C1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

FABP11, FABP3, Fatty acid binding protein 3, H FABP, M FABP, MDGI, O FABP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60280-1-PBS targets FABP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21483

Product name: Recombinant human FABP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-133 aa of BC007021

Sequence: MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA

相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
Calculated molecular weight 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC007021
Gene Symbol FABP3
Gene ID (NCBI) 2170
RRIDAB_2881398
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05413
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.

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