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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FABP2 Monoclonal antibody (67691-1-Ig)

    WB analysis using 67691-1-Ig (same clone as 67691-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using FABP2 Monoclonal antibody (67691-1-Ig)

    WB analysis of COLO 320 using 67691-1-Ig (same clone as 67691-1-PBS)

    WB result of FABP2 antibody (67691-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP2 transfected COLO 320 cells. This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FABP2 Monoclonal antibody (67691-1-Ig)

    WB analysis using 67691-1-Ig (same clone as 67691-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of rat small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of mouse small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of mouse small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of human small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of human small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of mouse colon using 67691-1-Ig (same clone as 67691-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IF Staining of mouse colon using 67691-1-Ig (same clone as 67691-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FABP2 antibody (67691-1-Ig, Clone: 2D11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FABP2 Monoclonal antibody, PBS Only

FABP2 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67691-1-PBS
Clone No.2D11G6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, ELISA

Synonyms

2D11G6, FABPI, Fatty acid binding protein 2, Fatty acid-binding protein 2, Fatty acid-binding protein, intestinal

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67691-1-PBS targets FABP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17620

Product name: Recombinant human FABP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-132 aa of BC069617

Sequence: MAFDSTWKVDRSENYDKFMEKMGVNIVKRKLAAHDNLKLTITQEGNKFTVKESSAFRNIEVVFELGVTFNYNLADGTELRGTWSLEGNKLIGKFKRTDNGNELNTVREIIGDELVQTYVYEGVEAKRIFKKD

相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 2, intestinal
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC069617
Gene Symbol FABP2
Gene ID (NCBI) 2169
RRIDAB_2882884
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP12104
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FABP2, also known as the intestinal fatty acid binding protein (I-FABP), is expressed in the absorptive intestinal villus cells. It is mainly involved in intracellular transport and intestinal absorption of lipids. FABP2 has been considered a marker of mucosal injury and ischemia and serum I-FABP level is used as a tissue damage indicator. In addition, it is a marker of differentiated intestinal epithelial cells.

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