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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FABP1 Polyclonal antibody (34024-1-AP)

    WB analysis using 34024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 34024-1-AP (FABP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using FABP1 Polyclonal antibody (34024-1-AP)

    IF Staining of mouse colon using 34024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using FABP1 Polyclonal antibody (34024-1-AP)

    IF Staining of mouse colon using 34024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat colon tissue using FABP1 Polyclonal antibody (34024-1-AP)

    IF Staining of rat colon using 34024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FABP1 Polyclonal antibody

FABP1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 34024-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, pig

Applications

WB, IF-P, ELISA

Synonyms

14 kDa selenium-binding protein, Fabpl, Fatty acid-binding protein 1, Fatty acid-binding protein, liver, L-FABP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat colon tissue, rat liver tissue, pig liver tissue
Positive IF-P detected inmouse colon tissue, rat colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

34024-1-AP targets FABP1 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat, pig samples.

Tested Reactivity mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg7277

Product name: Recombinant rat FABP1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 2-127 aa of NM_012556.2

Sequence: NFSGKYQVQSQENFEPFMKAMGLPEDLIQKGKDIKGVSEIVHEGKKVKLTITYGSKVIHNEFTLGEECELETMTGEKVKAVVKMEGDNKMVTTFKGIKSVTEFNGDTITNTMTLGDIVYKRVSKRI

相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 1, liver
Calculated molecular weight14 kDa
Observed molecular weight14 kDa
GenBank accession numberNM_012556.2
Gene Symbol Fabp1
Gene ID (NCBI) 24360
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP02692
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 have been found to be elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).

Protocols

Product Specific Protocols
IF protocol for FABP1 antibody 34024-1-APDownload protocol
WB protocol for FABP1 antibody 34024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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