Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HL-60 cells, Jurkat cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31841-1-AP targets FAAP100 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36385 Product name: Recombinant human C17orf70 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 524-730 aa of BC117139 Sequence: PVATFLETCREPGSQPAGPASLRAEYLPPSVASIKVSAELLRAALKDGHSGVPLCCATLQWLLAENAAVDVVRARALSSIQGVAPDGANVHLIVREVAMTDLCPAGPIQAVEIQVESSSLADICRAHHAVVGRMQTMVTEQAAQGSSAPDLRVQYLRQIHANHETLLREVQTLRDRLCTEDEASSCATAQRLLQVYRQLRHPSLILL 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 17 open reading frame 70 |
| Calculated molecular weight | 93 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC117139 |
| Gene Symbol | C17orf70 |
| Gene ID (NCBI) | 80233 |
| RRID | AB_3670125 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q0VG06 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FA core complex associated protein 100 (FAAP100, also known as C17orf70) is a component of the Fanconi anemia (FA), which plays a role in the FA-associated DNA damage response network as well as regulates Fanconi anemia group D2 protein (FANCD2) monoubiquitination and the stability of the FA core complex (PMID: 17396147). FAAP100 deletion leads to FA pathway inactivation, increases TRCs and cotranscriptional R-loops, and contributes to the collapse of replication forks and the generation of DNA damage (PMID: 37580696).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAAP100 antibody 31841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |