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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis using 67607-1-Ig (same clone as 67607-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of JAR cells using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis of JAR using 67607-1-Ig (same clone as 67607-1-PBS)

    JAR cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis of LNCaP using 67607-1-Ig (same clone as 67607-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human liver cancer using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human liver cancer using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 410x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human prostate cancer using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human prostate cancer using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using FAAH Monoclonal antibody (67607-1-Ig)

    IF Staining of LNCaP using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using FAAH antibody (67607-1-Ig, Clone: 3C5B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

FAAH Monoclonal antibody, PBS Only

FAAH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67607-1-PBS
Clone No.3C5B5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3C5B5, EC:3.1.1.-, EC:3.5.1.99, FAAH1, Fatty acid ester hydrolase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67607-1-PBS targets FAAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12720

Product name: Recombinant human FAAH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 236-579 aa of BC093632

Sequence: TDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAFLVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

相同性解析による交差性が予測される生物種
Full Name fatty acid amide hydrolase
Calculated molecular weight 579 aa, 63 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC093632
Gene Symbol FAAH
Gene ID (NCBI) 2166
RRIDAB_2882813
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO00519
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

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