Validation Data Gallery
Tested Applications
Positive WB detected in | MKN-45 cells, SGC-7901 cells, fetal human brain tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86020-2-RR targets FA2H in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | FA2H fusion protein Ag7717 相同性解析による交差性が予測される生物種 |
Full Name | fatty acid 2-hydroxylase |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 43 kDa |
GenBank accession number | BC002679 |
Gene Symbol | FA2H |
Gene ID (NCBI) | 79152 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q7L5A8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FA2H(Fatty acid 2-hydroxylase) required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. The deduced 372-amino acid protein has a calculated molecular mass of 42.8 kD .Both mouse and human FA2H have a C-terminal endoplasmic reticulum (ER) retention motif(PMID: 15658937).
Protocols
Product Specific Protocols | |
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WB protocol for FA2H antibody 86020-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |