Validation Data Gallery
Tested Applications
| Positive WB detected in | OVCAR-3 cells, SKOV-3 cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32696-1-AP targets F8A2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38529 Product name: Recombinant human F8A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-216 aa of NM_012151.3 Sequence: GDFLARYRLVSNKLKKRFLRKPNVAEAGEQFGQLGRELRAQECLPYAAWCQLAVARCQQALFHGPGEALALTEAARLFLRQERDARQRLVCPAAYGEPLQAAASALGAAVRLHLELGQPAAAAALCLELAAALRDLGQPAAAAGHFQRAAQLQLPQLPLAALQALGEAASCQLLARDYTGALAVFTRMQRLAREHGS 相同性解析による交差性が予測される生物種 |
| Full Name | coagulation factor VIII-associated (intronic transcript) 2 |
| Calculated molecular weight | 39kDa，371aa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | NM_012151.3 |
| Gene Symbol | F8A2 |
| Gene ID (NCBI) | 474383 |
| RRID | AB_3742713 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P23610 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
F8A2 (Coagulation Factor VIII Associated 2) is a protein associated with coagulation factor VIII, which is widely expressed in a variety of cell types, localized mainly in the cytoplasm and nucleus, and also co-localized with Huntington's protein (HTT). Although its specific function is not fully defined, F8A2 may play an important role in intracellular and vesicular transport. In addition, F8A2 co-localizes with ubiquitin in neuronal cells and may be involved in the pathological process of neurodegenerative diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for F8A2 antibody 32696-1-AP | Download protocol |
| WB protocol for F8A2 antibody 32696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |