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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Extra Range Prestained Protein Marker

Molecular Weight

10-310 kDa

Detection Method

Colorimetric, Fluorescence

Number of Markers

12

Stain Type

3 colors：Blue，Red，Green

Cat no : PL00003

Validation Data Gallery

  • <strong>Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions</strong><br>The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.

    Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions
    The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.

  • Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in figure.

    Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in figure.

  • 2 ul protein ladders were loaded in the 8%-18% gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75k Da and 310 kDa) and 680 nm (except 75k Da and 310 kDa) range.

    2 ul protein ladders were loaded in the 8%-18% gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75k Da and 310 kDa) and 680 nm (except 75k Da and 310 kDa) range.

Product Information

The PL00003 Extra Range Prestained Protein Marker is a ready-to-use three-color protein standard with 12 prestained proteins covering a wide range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer. The PL00003 Extra Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins. The PL00003 Extra Range Prestained Protein Marker is also suitable for fluorescence WB detection.


Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.

Usage

The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method

Colorimetric

Molecular Weight

~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180, 245, ~310 kDa

Number of Markers

12

Size Range

10 to 310

Publications

ApplicationTitle

Cell Death Dis

EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli.

Authors - Shouyang Song
View Article

CNS Neurosci Ther

A novel NMDA receptor modulator: the antidepressant effect and mechanism of GW043

Authors - Murezati Tiliwaerde
View Article

Chin Med

Isoforskolin modulates AQP4-SPP1-PIK3C3 related pathway for chronic obstructive pulmonary disease via cAMP signaling

Authors - Haochang Lin
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Rocio (Verified Customer) (03-19-2024)

Works pretty well, all the colors are easy visible!

  • Applications: Western Blot