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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    WB analysis using 84490-2-RR (same clone as 84490-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84490-2-RR (EpCAM/TROP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01344-1

    Sandwich ELISA standard curve of MP01344-1

    Sandwich ELISA standard curve of MP01344-1, Mouse EPCAM/CD326 Recombinant Matched Antibody Pair - PBS only. 84490-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1883. 84490-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

  • Sample test of MP01344-1

    Sample test of MP01344-1

    The mean EPCAM/CD326 concentration was determined to be 76,517.6 pg/mL in mouse kidney tissue extract based on a 3.1 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IHC staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IHC staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IHC staining of mouse small intestine using 84490-2-RR (same clone as 84490-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IHC staining of mouse small intestine using 84490-2-RR (same clone as 84490-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IF Staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using EPCAM/CD326 antibody (84490-2-RR, Clone: 241851E11 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IF Staining of mouse small intestine using 84490-2-RR (same clone as 84490-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using EPCAM/CD326 antibody (84490-2-RR, Clone: 241851E11 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01344-1

    Cytometric bead array standard curve of MP01344-1

    Cytometric bead array standard curve of MP01344-1, MOUSE EPCAM/CD326 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84490-2-PBS. Detection antibody: 84490-3-PBS. Standard: Eg1883. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP01344-2

    Cytometric bead array standard curve of MP01344-2

    Cytometric bead array standard curve of MP01344-2, MOUSE EPCAM/CD326 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84490-2-PBS. Detection antibody: 84490-1-PBS. Standard: Eg1883. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84490-2-RR

    Affinity and Kinetic Characterization of 84490-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84490-2-RR against Mouse EPCAM/CD326 were performed. The affinity constant is 0.779 nM.

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Mouse EPCAM/CD326 Recombinant antibody, PBS Only (Capture)

EPCAM/CD326 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84490-2-PBS
Clone No.241851E11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314, CD326

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84490-2-PBS targets EPCAM/CD326 as part of a matched antibody pair:

MP01344-1: 84490-2-PBS capture and 84490-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01344-2: 84490-2-PBS capture and 84490-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name epithelial cell adhesion molecule
Calculated molecular weight35 kDa
Observed molecular weight35 kDa, 40 kDa
GenBank accession numberNM_008532.2
Gene Symbol Epcam
Gene ID (NCBI) 17075
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99JW5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)