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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Endothelin 3 Polyclonal antibody (27871-1-AP)

    WB analysis using 27871-1-AP (same clone as 27871-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27871-1-AP (Endothelin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Endothelin 3 Polyclonal antibody (27871-1-AP)

    IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Endothelin 3 Polyclonal antibody (27871-1-AP)

    IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Endothelin 3 Polyclonal antibody (27871-1-AP)

    IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Endothelin 3 Polyclonal antibody (27871-1-AP)

    IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Endothelin 3 Polyclonal antibody (27871-1-AP)

    IF Staining of NIH/3T3 using 27871-1-AP (same clone as 27871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Endothelin 3 antibody (27871-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using Endothelin 3 Polyclonal antibody (27871-1-AP)

    IF Staining of BxPC-3 using 27871-1-AP (same clone as 27871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Endothelin 3 antibody (27871-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

Endothelin 3 Polyclonal antibody, PBS Only

Endothelin 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27871-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EDN3, Endothelin-3, ET 3, ET3, ET-3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27871-1-PBS targets Endothelin 3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27393

Product name: Recombinant human EDN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-198 aa of BC053866

Sequence: SQSGDAGRRGVSQAPTAARSEGDCEETVAGPGEETVAGPGEGTVAPTALQGPSPGSPGQEQAAEGAPEHHRSRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRGSFRGKRSAGPLPGNLQLSHRPHLRCACVGRYDKACLHFCTQTLDVSSNSRTAEKTDKEEEGKVE

相同性解析による交差性が予測される生物種
Full Name endothelin 3
Observed molecular weight 28 kDa
GenBank accession numberBC053866
Gene Symbol Endothelin 3
Gene ID (NCBI) 1908
RRIDAB_3086000
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP14138
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Endothelin 3 (EDN3) is also named as ET-3 and Preproendothelin-3 (PPET3). Endothelin 3 belongs to the endothelin/sarafotoxin family. It reported downregulation of EDN3 as a result of methylation of its promoter in breast cancer, and EDN3 level was also decreased in cervical cancer (PMID: 36542159). ET-3 is the most abundant ET peptide in the rat brain, mainly localized to neurons and glia of the neostriatum, hypothalamic nuclei, hippocampus, and Purkinje cells of the cerebellum and medulla oblongata (PMID: 32589590).

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