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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 750 Anti-Human Endoglin/CD105 Rabbi (CL750-98013)

    FC experiment of human PBMCs using CL750-98013

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (CL750-98013, Clone:230047E5), or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 750 Anti-Human Endoglin/CD105 Rabbi (CL750-98013)

    FC experiment of human PBMCs using CL750-98013

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (CL750-98013, Clone:230047E5)(red), or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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CoraLite® Plus 750 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody

Endoglin/CD105 Recombinant Antibody for FC
Cat No. CL750-98013
Clone No.230047E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD105, 230047E5, HHT1, ENG, Endoglin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-98013 targets Endoglin/CD105 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name endoglin
Calculated molecular weight 71 kDa
GenBank accession numberBC014271
Gene Symbol CD105
Gene ID (NCBI) 2022
RRIDAB_3673784
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17813
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 Endoglin/CD105 antibody CL750-98013Download protocol
Standard Protocols
Click here to view our Standard Protocols