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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using EYA2 Recombinant monoclonal antibody (86046-2-RR)

    WB analysis of Y79 using 86046-2-RR

    Y79 cells were subjected to SDS PAGE followed by western blot with 86046-2-RR (EYA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of IMR-32 cells using EYA2 Recombinant monoclonal antibody (86046-2-RR)

    WB analysis of IMR-32 using 86046-2-RR

    IMR-32 cells were subjected to SDS PAGE followed by western blot with 86046-2-RR (EYA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

EYA2 Recombinant monoclonal antibody

EYA2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86046-2-RR
Clone No.250437D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EAB1, EC:3.1.3.48

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inIMR-32 cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86046-2-RR targets EYA2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag29403

Product name: Recombinant human EYA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 241-583 aa of BC013882

Sequence: LSYGSSFSTSPTGQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFGRKAVYVVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL

相同性解析による交差性が予測される生物種
Full Name eyes absent homolog 2 (Drosophila)
Calculated molecular weight 64 kDa
Observed molecular weight60 kDa
GenBank accession numberBC013882
Gene Symbol EYA2
Gene ID (NCBI) 2139
RRIDAB_3744576
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00167
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. This recombinant antibody can not detect the modified isform with the molecular weight of 70 kda, compared with 11314-1-AP.

Protocols

Product Specific Protocols
WB protocol for EYA2 antibody 86046-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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