Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human prostate hyperplasia tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
24470-1-AP targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19768 Product name: Recombinant human EXOSC9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC142978 Sequence: MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGAYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLE 相同性解析による交差性が予測される生物種 |
| Full Name | exosome component 9 |
| Calculated molecular weight | 456 aa, 51 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC142978 |
| Gene Symbol | EXOSC9 |
| Gene ID (NCBI) | 5393 |
| RRID | AB_2879559 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06265 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| IHC protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| WB protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication | ||
G3 (Bethesda) Humanized Saccharomyces cerevisiae provides a facile and effective tool to identify damaging human variants that cause exosomopathies | ||