Validation Data Gallery
Tested Applications
| Positive WB detected in | A2780 cells, HEK-293T cells, PC-3 cells, NIH/3T3 cells, mouse spleen tissue |
| Positive IP detected in | A2780 cells |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 16 publications below |
| IF | See 2 publications below |
Product Information
15062-1-AP targets EXOSC3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7065 Product name: Recombinant human EXOSC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC002437 Sequence: MAEPASVAAESLAGSRARAARTVLGQVVLPGEELLLPEQEDAEGPGGAVERPLSLNARACSRVRVVCGPGLRRCGDRLLVTKCGRLRHKEPGSGSGGGVYWVDSQQKRYVPVKGDHVIGIVTAKSGDIFKVDVGGSEPASLSYLSFEGATKRNRPNVQVGDLIYGQFVVANKDMEPEMVCIDSCGRANGMGVIGQDGLLFKVTLGLIRKLLAPDCEIIQEVGKLYPLEIVFGMNGRIWVKAKTIQQTLILANILEACEHMTSDQRKQIFSRLAES 相同性解析による交差性が予測される生物種 |
| Full Name | exosome component 3 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC002437 |
| Gene Symbol | EXOSC3 |
| Gene ID (NCBI) | 51010 |
| RRID | AB_2278183 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQT5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNA exosomes are multi-subunit complexes conserved throughout evolution, and they are emerging as the major cellular machinery for processing, surveillance and turnover of a diverse spectrum of coding and noncoding RNA substrates essential for viability[PMID:22544365]. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts [PMID:11782436]. EXOSC3 is a non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of Rnase PH-domain subunits through contacts with EXOSC9 and EXOSC5 [PMID:21255825].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EXOSC3 antibody 15062-1-AP | Download protocol |
| IHC protocol for EXOSC3 antibody 15062-1-AP | Download protocol |
| IP protocol for EXOSC3 antibody 15062-1-AP | Download protocol |
| WB protocol for EXOSC3 antibody 15062-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia. | ||
Mol Cell Large-scale tethered screen of RNA-binding proteins reveals novel regulators of poly(A) site selection. | ||
Mol Cell A nuclear RNA degradation code is recognized by PAXT for eukaryotic transcriptome surveillance | ||
Mol Cell Integrator is a genome-wide attenuator of non-productive transcription.
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