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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOC8 Polyclonal antibody (31523-1-AP)

    WB analysis using 31523-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31523-1-AP (EXOC8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EXOC8 Polyclonal antibody (31523-1-AP)

    IF Staining of A431 using 31523-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EXOC8 antibody (31523-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

EXOC8 Polyclonal antibody

EXOC8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31523-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SEC84, Exo84p, EXO84

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHuH-7 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31523-1-AP targets EXOC8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOC8 fusion protein Ag33300 相同性解析による交差性が予測される生物種
Full Name exocyst complex component 8
Calculated molecular weight 725 aa, 82 kDa
Observed molecular weight82 kDa
GenBank accession numberBC035763
Gene Symbol EXOC8
Gene ID (NCBI) 149371
RRIDAB_3670020
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8IYI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXOC8 is a subunit of the exocyst complex involved in docking exocytic vesicles with fusion sites on the plasma membrane. Truncated EXOC8 protein leads to the improper assembly of the exocyst complex, thus resulting in the accumulation of neurotransmitters and other excretory vesicles in the cell. It is found to play an essential role in normal cortical development and its perturbation causes complex brain disorders. EXOC8 mutations are linked to neurodevelopmental disorders with microcephaly, seizures, and brain atrophy (PMID: 35460391, PMID: 36344539).

Protocols

Product Specific Protocols
WB protocol for EXOC8 antibody 31523-1-APDownload protocol
IF protocol for EXOC8 antibody 31523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols