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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOC5 Polyclonal antibody (17593-1-AP)

    WB analysis using 17593-1-AP (same clone as 17593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17593-1-AP (EXOC5 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using EXOC5 Polyclonal antibody (17593-1-AP)

    IP experiment of mouse brain using 17593-1-AP (same clone as 17593-1-PBS)

    IP result of anti-EXOC5 (IP:17593-1-AP, 3ug; Detection:17593-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using EXOC5 Polyclonal antibody (17593-1-AP)

    IHC staining of human testis using 17593-1-AP (same clone as 17593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using EXOC5 Polyclonal antibody (17593-1-AP)

    IHC staining of human testis using 17593-1-AP (same clone as 17593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using EXOC5 Polyclonal antibody (17593-1-AP)

    IHC staining of human brain using 17593-1-AP (same clone as 17593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using EXOC5 Polyclonal antibody (17593-1-AP)

    IHC staining of human brain using 17593-1-AP (same clone as 17593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EXOC5 Polyclonal antibody (17593-1-AP)

    IF Staining of HepG2 using 17593-1-AP (same clone as 17593-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using EXOC5 antibody 17593-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EXOC5 Polyclonal antibody, PBS Only

EXOC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17593-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Exocyst complex component 5, Exocyst complex component Sec10, hSec10, SEC10, SEC10L1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17593-1-PBS targets EXOC5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11524

Product name: Recombinant human SEC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 359-708 aa of BC041126

Sequence: SRSAMILQRYYDSKNHQKRSIGTGGIQDLKERIRQRTNLPLGPSIDTHGETFLSQEVVVNLLQETKQAFERCHRLSDPSDLPRNAFRIFTILVEFLCIEHIDYALETGLAGIPSSDSRNANLYFLDVVQQANTIFHLFDKQFNDHLMPLISSSPKLSECLQKKKEIIEQMEMKLDTGIDRTLNCMIRQMKHILAAEQKKTDFKPEDENNVLIQYTNACVKVCAYVRKQVEKIKNSMDGKNVDTVLMELGVRFHRLIYEHLQQYSYSCMGGMLAICDVAEYRKCAKDFKIPMVLHLFDTLHALCNLLVVAPDNLKQVCSGEQLANLDKNILHSFVQLRADYRSARLARHFS

相同性解析による交差性が予測される生物種
Full Name exocyst complex component 5
Calculated molecular weight 708 aa, 82 kDa
Observed molecular weight 70-77 kDa
GenBank accession numberBC041126
Gene Symbol EXOC5
Gene ID (NCBI) 10640
RRIDAB_2101582
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO00471
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The complex is also essential for the biogenesis of epithelial cell surface polarity.

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