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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EXOC3L2 Monoclonal antibody

EXOC3L2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C1C2

Cat no : 66537-1-Ig

Synonyms

EXOC3L2, XTP7

Validation Data Gallery

Applications:
  • WB analysis of HUVEC using 66537-1-Ig

    WB analysis of HUVEC using 66537-1-Ig

    HUVEC, HEK-293 and U-251 cells were subjected to SDS PAGE followed by western blot with 66537-1-Ig (EXOC3L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 66537-1-Ig

    IF Staining of HUVEC using 66537-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using EXOC3L2 antibody (66537-1-Ig, Clone: 3C1C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHUVEC cells
Positive IF detected inHUVEC cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66537-1-Ig targets EXOC3L2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EXOC3L2 fusion protein Ag26912 相同性解析による交差性が予測される生物種
Full Name exocyst complex component 3-like 2
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC104947
Gene symbol EXOC3L2
Gene ID (NCBI) 90332
RRIDAB_2881900
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The exocyst is a protein complex that ensures spatial targeting of exocytotic vesicles to the plasma membrane. Exocyst complex component 3-like 2 (EXOC3L2) associates with the exocyst complex and mediates directional migration of endothelial cells (PMID: 21566143). The gene of human EXOC3L2 maps to chromosome 19q13.32 and encodes a deduced 409-amino acid protein with a calculated molecular mass of about 46 kDa. Mouse Exoc3l2 gene encodes an approximately 250-amino acid protein that lacks the N-terminal domain of human EXOC3L2. Mutations in EXOC3L2 gene may be associated with Alzheimer's disease.

Protocols

Product Specific Protocols
WB protocol for EXOC3L2 antibody 66537-1-IgDownload protocol
IF protocol for EXOC3L2 antibody 66537-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Genet

Biallelic mutations in EXOC3L2 cause a novel syndrome that affects the brain, kidney and blood.

Authors - Adel Shalata
View Article