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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXO1/Exonuclease 1 Recombinant monoclonal antibody (86445-1-RR)

    WB analysis using 86445-1-RR (same clone as 86445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86445-1-RR (EXO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86445-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02742-1

    Sandwich ELISA sample test of MP02742-1

    The mean EXO1/Exonuclease 1 concentration was determined to be 4.80 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02742-1

    Sandwich ELISA standard curve of MP02742-1

    Sandwich ELISA standard curve of MP02742-1, Human EXO1/Exonuclease 1 Recombinant Matched Antibody Pair - PBS only. 86445-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9253. 86445-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EXO1/Exonuclease 1 Recombinant monoclonal antibody (86445-1-RR)

    IF Staining of HEK-293 using 86445-1-RR (same clone as 86445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EXO1 antibody (86445-1-RR, Clone: 251210D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86445-1-PBS in a different storage buffer formulation.

EXO1/Exonuclease 1 Recombinant monoclonal antibody, PBS Only (Capture)

EXO1/Exonuclease 1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86445-1-PBS
Clone No.251210D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

EXO1, EC:3.1.-.-, EXOI, Exonuclease 1, Exonuclease I

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86445-1-PBS targets EXO1/Exonuclease 1 as part of a matched antibody pair:

MP02742-1: 86445-1-PBS capture and 86445-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9253

Product name: Recombinant human EXO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 496-846 aa of BC007491

Sequence: AVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREGACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLCLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ

相同性解析による交差性が予測される生物種
Full Name exonuclease 1
Calculated molecular weight 94 kDa
Observed molecular weight 115-120 kDa
GenBank accession numberBC007491
Gene Symbol EXO1
Gene ID (NCBI) 9156
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UQ84
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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