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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

EXD2 Polyclonal antibody

EXD2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20138-1-AP

Synonyms

C14orf114, EXD2, EXDL2

Validation Data Gallery

Applications:
  • WB analysis of mouse lung using 20138-1-AP

    WB analysis of mouse lung using 20138-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse pancreas using 20138-1-AP

    WB analysis of mouse pancreas using 20138-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 20138-1-AP

    WB analysis of mouse brain using 20138-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 20138-1-AP

    IP experiment of mouse lung using 20138-1-AP

    IP result of anti-EXD2 (IP:20138-1-AP, 3ug; Detection:20138-1-AP 1:300) with mouse lung tissue lysate 5160ug.

  • IHC staining of human testis using 20138-1-AP

    IHC staining of human testis using 20138-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20138-1-AP (EXD2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 20138-1-AP

    IHC staining of human testis using 20138-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20138-1-AP (EXD2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20138-1-AP

    IF Staining of HepG2 using 20138-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EXD2 antibody (20138-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse lung tissue, mouse brain tissue, mouse pancreas tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20138-1-AP targets EXD2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXD2 fusion protein Ag14090 相同性解析による交差性が予測される生物種
Full Name exonuclease 3'-5' domain containing 2
Calculated molecular weight 70 kD aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC001962
Gene symbol EXD2
Gene ID (NCBI) 55218
RRIDAB_10665419
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EXD2 antibody 20138-1-APDownload protocol
IHC protocol for EXD2 antibody 20138-1-APDownload protocol
IF protocol for EXD2 antibody 20138-1-APDownload protocol
IP protocol for EXD2 antibody 20138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination.

Authors - Xikui Sun
  • KO Validated
View Article
mouseWB,IF

Andrology

Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes

Authors - Opeyemi Olotu
View Article