Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, mouse brain tissue, mouse pancreas tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
20138-1-AP targets EXD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EXD2 fusion protein Ag14090 相同性解析による交差性が予測される生物種 |
| Full Name | exonuclease 3'-5' domain containing 2 |
| Calculated molecular weight | 70 kD aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC001962 |
| Gene Symbol | EXD2 |
| Gene ID (NCBI) | 55218 |
| RRID | AB_10665419 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EXD2 (Exonuclease 3'-5' Domain Containing 2), is a protein with exonuclease activity of 3'-5' nucleic acid, which is involved in many biological processes in cells, including DNA repair, RNA processing and mitochondrial translation. EXD2 protein is mainly located in mitochondrial outer membrane, intermediate fiber cytoskeleton and DNA damage site. This protein may be a potential drug target in some cancers. For example, in ALT (telomere selective elongation) dependent cancer, the co-elimination of EXD2 with BLM, DNA2 or POLD3 may mediate synthetic lethality. In addition, EXD2 inhibitors may exert anticancer effects by inhibiting mitochondrial translation and metabolic reprogramming. It has two isoforms with molecular weights of 56 kDa and 70 kDa respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EXD2 antibody 20138-1-AP | Download protocol |
| IHC protocol for EXD2 antibody 20138-1-AP | Download protocol |
| IF protocol for EXD2 antibody 20138-1-AP | Download protocol |
| IP protocol for EXD2 antibody 20138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination.
| ||
Andrology Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes |