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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EWS Polyclonal antibody (55191-1-AP)

    WB analysis using 55191-1-AP (same clone as 55191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using EWS Polyclonal antibody (55191-1-AP)

    WB analysis of mouse testis using 55191-1-AP (same clone as 55191-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using EWS Polyclonal antibody (55191-1-AP)

    WB analysis of Jurkat using 55191-1-AP (same clone as 55191-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EWS Polyclonal antibody (55191-1-AP)

    IHC staining of human colon cancer using 55191-1-AP (same clone as 55191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using EWS Polyclonal antibody (55191-1-AP)

    IHC staining of human small intestine using 55191-1-AP (same clone as 55191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using EWS Polyclonal antibody (55191-1-AP)

    IHC staining of human small intestine using 55191-1-AP (same clone as 55191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EWS Polyclonal antibody (55191-1-AP)

    IHC staining of human colon cancer using 55191-1-AP (same clone as 55191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

EWS Polyclonal antibody, PBS Only

EWS Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55191-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EWSR1, EWS oncogene, RNA binding protein EWS, RNA-binding protein EWS

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55191-1-PBS targets EWS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name Ewing sarcoma breakpoint region 1
Calculated molecular weight 68 kDa
Observed molecular weight68-70 kDa
GenBank accession numberNM_005243
Gene Symbol EWS
Gene ID (NCBI) 2130
RRIDAB_10860907
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01844
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).

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