Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells, RT-4 cells, U-251 cells, rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32934-1-AP targets EVI5L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35996 Product name: Recombinant human EVI5L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 421-620 aa of BC014111 Sequence: VVRQQCSSAAEDLQKAQSTIRQLQEQQENPRLTEDFVSHLETELEQSRLRETETLGALREMQDKVLDMEKRNSSLPDENNVAQLQEELKALKVREGQAVASTRELKLQLQELSDTWQAHLARGGRWKESPRKLVVGELQDELMSVRLREAQALAEGRELRQRVVELETQDHIHRNLLNRVEAERAALQEKLQYLAAQNKG 相同性解析による交差性が予測される生物種 |
| Full Name | ecotropic viral integration site 5-like |
| Observed molecular weight | 91,92 kDa |
| GenBank accession number | BC014111 |
| Gene Symbol | EVI5L |
| Gene ID (NCBI) | 115704 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96CN4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Evi5l (Ecotropic Viral Integration Site 5-Like), encodes a RabGAP that is known to block the formation of cilia when overexpressed (PMID: 26136363). EVI5L has ubiquitously low expression but is slightly higher in the testis and fetal brain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EVI5L antibody 32934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |