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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse stomach tissue using EVI5 Recombinant antibody (84738-2-RR)

    IHC staining of mouse stomach using 84738-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84738-2-RR (EVI5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EVI5 Recombinant antibody (84738-2-RR)

    IF Staining of U2OS using 84738-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EVI5 antibody (84738-2-RR, Clone: 241918D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using EVI5 Recombinant antibody (84738-2-RR)

    FC experiment of U2OS using 84738-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug EVI5 Recombinant antibody (84738-2-RR, Clone:241918D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84738-2-RR

    Affinity and Kinetic Characterization of 84738-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84738-2-RR against Human EVI5 were performed. The affinity constant is 1.11 nM.

EVI5 Recombinant monoclonal antibody

EVI5 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84738-2-RR
Clone No.241918D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

241918D11, NB4S

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84738-2-RR targets EVI5 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26739

Product name: Recombinant human EVI5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-80 aa of BC156793

Sequence: MVTNKMTAAFRNPSGKQVATDKVAEKLSSTLSWVKNTVSHTVSQMASQVASPSTSLHTTSSSTTLSTPALSPSSPSQLSP

相同性解析による交差性が予測される生物種
Full Name ecotropic viral integration site 5
Calculated molecular weight 810 aa, 93 kDa
GenBank accession numberBC156793
Gene Symbol EVI5
Gene ID (NCBI) 7813
RRIDAB_3672122
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO60447
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EVI5 functions as a regulator of cell cycle progression by stabilizing the FBXO5 protein and promoting cyclin-A accumulation during interphase. It may play a role in cytokinesis (PMID: 16439210).

Protocols

Product Specific Protocols
IF protocol for EVI5 antibody 84738-2-RRDownload protocol
IHC protocol for EVI5 antibody 84738-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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