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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EVI2B Recombinant antibody (85943-1-RR)

    WB analysis using 85943-1-RR (same clone as 85943-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85943-1-RR (EVI2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85943-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using EVI2B Recombinant antibody (85943-1-RR)

    IF Staining of THP-1 using 85943-1-RR (same clone as 85943-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using EVI2B antibody (85943-1-RR, Clone: 250344C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85943-1-PBS in a different storage buffer formulation.

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EVI2B Recombinant antibody, PBS Only

EVI2B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85943-1-PBS
Clone No.250344C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CD361, D17S376, EVDB, EVI 2B, EVI-2B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85943-1-PBS targets EVI2B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EVI2B fusion protein Eg3709 相同性解析による交差性が予測される生物種
Full Name ecotropic viral integration site 2B
Calculated molecular weight 448 aa, 49 kDa
Observed molecular weight60 kDa
GenBank accession numberBC005926
Gene Symbol EVI2B
Gene ID (NCBI) 2124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP34910
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ecotropic viral integration site 2B protein (EVI2B) is a type I transmembrane protein embedded within intron 27b of the neurofibromatosis type 1 (NF1) gene. It is transcribed in the opposite direction to the NF1 gene (PMID: 37584733). EVI2B is a downstream target of CCAAT/enhancer binding protein alpha (C/EBPα), which is crucial for myeloid differentiation and the function of hematopoietic progenitors (PMID: 36830696). This protein is highly expressed in mature granulocytes and is involved in the regulation of immune cell maturation (PMID: 28186500).