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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using EVI2B Polyclonal antibody (24891-1-AP)

    WB analysis of HepG2 using 24891-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24891-1-AP (EVI2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using EVI2B Polyclonal antibody (24891-1-AP)

    WB analysis of A549 using 24891-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24891-1-AP (EVI2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using EVI2B Polyclonal antibody (24891-1-AP)

    IHC staining of human tonsillitis using 24891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24891-1-AP (EVI2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using EVI2B Polyclonal antibody (24891-1-AP)

    IHC staining of human tonsillitis using 24891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24891-1-AP (EVI2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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EVI2B Polyclonal antibody

EVI2B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24891-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Protein EVI2B, EVI-2B, EVI 2B, EVDB, D17S376

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, A549 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

24891-1-AP targets EVI2B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EVI2B fusion protein Ag19511 相同性解析による交差性が予測される生物種
Full Name ecotropic viral integration site 2B
Calculated molecular weight 448 aa, 49 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC005926
Gene Symbol EVI2B
Gene ID (NCBI) 2124
RRIDAB_2879781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EVI2B antibody 24891-1-APDownload protocol
IHC protocol for EVI2B antibody 24891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma.

Authors - Bingsheng Yang
View Article
humanIHC

Exp Biol Med (Maywood)

Analysis of scRNA-seq and bulk RNA-seq demonstrates the effects of EVI2B or CD361 on CD8+ T cells in osteosarcoma

Authors - Tianyu Xie
View Article