Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86935-1-RR targets EVI1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag40447 Product name: Recombinant human EVI1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 813-1052 aa of BC130520 Sequence: HENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFTDSLKMRKMEDNQYSEAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTSHSSSNVWHSMARAAAESSAIQSISHV 相同性解析による交差性が予測される生物種 |
| Full Name | ecotropic viral integration site 1 |
| Calculated molecular weight | 1239 aa, 139 kDa |
| Observed molecular weight | 118 kDa, 140 kDa |
| GenBank accession number | BC130520 |
| Gene Symbol | EVI1 |
| Gene ID (NCBI) | 2122 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q03112 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Evi1 (ecotropic viral integration site 1) gene is an important proto-oncogene, which encodes a transcription factor with zinc finger structure, and this protein regulates the expression of other genes by binding to specific DNA sequences. EVI1 is highly expressed in hematopoietic stem cells (HSC) of human embryo and adult bone marrow, and rapidly down-regulated during differentiation. EVI1 knockout will lead to proliferation and regeneration defects of mouse HSC, accompanied by low expression of GATA2. Rebuilding the expression of EVI1 can restore the ability of proliferation and differentiation of mouse HSC by up-regulating GATA2. The two zinc finger domains of EVI1 can independently bind DNA and regulate different target genes, and they cooperate with FOS protein to jointly regulate cell adhesion, proliferation, colony formation and other processes. In advanced ovarian cancer, EVI1 and AP1 (activator protein 1, FOS is one of its main members) are both expressed abundantly.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EVI1 antibody 86935-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |