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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ETV6 Monoclonal antibody (67579-1-Ig)

    WB analysis of Jurkat using 67579-1-Ig (same clone as 67579-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ETV6 Monoclonal antibody (67579-1-Ig)

    WB analysis of K-562 using 67579-1-Ig (same clone as 67579-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using ETV6 Monoclonal antibody (67579-1-Ig)

    WB analysis of THP-1 using 67579-1-Ig (same clone as 67579-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using ETV6 Monoclonal antibody (67579-1-Ig)

    WB analysis of U2OS using 67579-1-Ig (same clone as 67579-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ETV6 Monoclonal antibody (67579-1-Ig)

    WB analysis of A431 using 67579-1-Ig (same clone as 67579-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ETV6 Monoclonal antibody (67579-1-Ig)

    IHC staining of human breast cancer using 67579-1-Ig (same clone as 67579-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ETV6 Monoclonal antibody (67579-1-Ig)

    IHC staining of human breast cancer using 67579-1-Ig (same clone as 67579-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ETV6 Monoclonal antibody (67579-1-Ig)

    IHC staining of human lung cancer using 67579-1-Ig (same clone as 67579-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ETV6 Monoclonal antibody (67579-1-Ig)

    IHC staining of human lung cancer using 67579-1-Ig (same clone as 67579-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

ETV6 Monoclonal antibody, PBS Only

ETV6 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67579-1-PBS
Clone No.2A5A2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

67579-1-Ig

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67579-1-PBS targets ETV6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30054

Product name: Recombinant human ETV6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 150-312 aa of BC043399

Sequence: QNHEEDNCVQRTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSPDPEQRPLRSPLDNMIRRLSPAERAQGPRPHQENNHQESYPLSVSPMENNHCPASSESHPKPSSPRQESTRVIQLMPSPIMHPLILNPRHSVDFKQSRLSEDGLHREGKPINLSHREDL

相同性解析による交差性が予測される生物種
Full Name ets variant 6
Calculated molecular weight 452 aa, 53 kDa
Observed molecular weight60 kDa
GenBank accession numberBC043399
Gene Symbol ETV6
Gene ID (NCBI) 2120
RRIDAB_2918493
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP41212
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ETV6(ETS Variant Transcription Factor 6), also known as TEL, is a Protein Coding gene. ETV6 is a member of the ETS family of transcription factors encoded by the ETS variant 6 gene (ETV6), located on chromosome 12p13.2. ETV6 protein contains 3 domains: a) the pointed N-terminal (i.e. PNT) domain which forms oligomer partners with itself as well as other transcription factors (e.g. FLI1) and is required for ETV6's transcriptional repressing activity; b) the central regulatory domain; and c) the C-terminal DNA-binding domain(PMID: 22578774). ETV6 is highly expressed in early hematopoietic progenitor cells(HPCs) and is essential for hematopoiesis in the bone marrow(PMID: 31248877).

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